Pengaruh pemberian ekstrak daun alfalfa (Medicago sativa) terhadap kadar glukosa darah tikus hiperkolesterolemia

Joyeti Darni, S.Gz., M.Gizi

Abstract

Background: Hypercholesterolemic as an indicator of cardiovascular disease is a major cause of morbidity and mortality in the world. Increased oxidative stress in hypercholesterolemic triggers lipid peroxidation effect directly on the cell membrane damage and initiate various diseases. The use of natural materials with relatively few side effects is an  hyperglycemic management alternative. Alfalfa stimulation of insulin secretion, and also improves insulin function by reducing plasma glucose. Experimental studies showed that Alfalfa leaf extract had an effective hypoglycemic activity. Objectives: To determine the effect of Alfalfa leaf extract on blood glucose level of hypercholesterolemic rats. Method: Pre-posttest randomized control group used twenty eight male Sprague Dawley rats were randomly divided into four groups, Sprague Dawley rats without treatment (control), Sprague Dawley rats with extract at dose 20 mg/200 g/d (X1), dose 40 mg/200 g/d (X2), dose 60 mg/200g/d (X3) for 21 days after the rats got hypercholesterolemic. Blood glucose level was measured by GOD-PAP method. Hypothesis test was analyzed by One Way Anova continued by Post hoc LSD test. Results: Alfalfa extract significant decrease in serum total cholesterol (p<0.000). Conclusion: The treatment of Alfalfa (Medicago sativa) leaf extract gives an effect of lower blood glucose level.

Keywords

Alfalfa; hyperglycemic; blood glucose

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References

Purnamasari, D. Diagnosis dan Klasifikasi Diabetes Mellitus. Jakarta: Interna Publishing; 2010

Shahab, A. Komplikasi Kronik DM Penyakit Jantung Koroner. Jakarta:Interna Publishing; 2010.

Amin KA, El-Twab TM. Oxidative markers, nitric oxide and homocysteine ateration in hypercholesterolemic rats: role of atorvastatine and cinnamon. Int J Clin Med. 2009. 2:2.54-65.

World Health Organization. World Health Statistic 2013. Italy: 2013.

Cahyono, J.B.S.B. Gaya Hidup dan Penyakit Modern. Yogyakarta: Kanisius; 2008.

Ekawati E. Hubungan Kadar Glukosa Darah Terhadap Hypertriglyceridemia Pada Penderita Diabetes Mellitus. Prosiding Seminar Nasional Kimia Unesa. Universitas Negeri Surabaya. 2012.

Mahendra, Krisnatuti D, Tobing A, Boy. Care Your Self Diabetes Mellitus. Jakarta: Penebar Plus. 2008

Subashini R and Rajadurai M. Evaluation of cardioprotective efficacy of Nelumbo nucifera Leaf extract on isoproterenol-induced myocardial infarction in Wistar rats. International Journal of Pharmacology and Biological Sciences. 2011; 2(4): 34-45.

Astawan, Made., Kasih, Andreas L. (2008). Khasiat warna-warni Makanan. Gramedia Pustaka Utama, Jakarta.

Subantoro, R., 2009, Mengenal Karakter Alfalfa (Medicago sativa L.), Mediagro Jurnal Ilmu Pertanian, 5, 2, 50-62

Laksmonowati, E., 2005, Uji Efek Antidiabetik Ekstrak Etanolik Daun dan Batang Alfalfa (Medicago sativa L.) pada Tikus Putih Jantan Galur Wistar yang Dibebani Glukosa dan Identifikasi Senyawa Aktifnya, Skripsi, Fakultas Farmasi Universitas Wahid Hasyim Semarang

Masomeh Khosravi Farsani, Esmaiel Amraie, Peyman Kavian, and Mahtab Keshvari4. Effects of aqueous extract of alfalfa on hyperglycemia and dyslipidemia in alloxan-induced diabetic Wistar rats. Interv Med Appl Sci. 2016 Sep; 8(3): 103–108. doi: [10.1556/1646.8.2016.3.5]

Zargari A. (1996): Therapeutic Plants (1st ed.). Tehran University Press, Tehran, pp. 642–646

Hong Y-H, Chao W-W, Chen M-L, Lin B-F: Ethyl acetate extracts of alfalfa (Medicago sativa L.) sprouts inhibit lipopolysaccharide-induced inflammation in vitro and in vivo. J Biomed Sci 16, 64 (2009)

Asgary S, Moshtaghian J, Hosseini M, Siadat H: Effects of alfalfa on lipoproteins and fatty streak formation in hypercholesterolemic rabbits. Pak J Pharm Sci 21, 460–464 (2008)

Mehranjani M, Shariatzadeh M, Desfulian A, Noori M, Abnosi M, Moghadam Z: Effects of Medicago sativa on nephropathy in diabetic rats. Indian J Pharm Sci 69, 768–772 (2007)

Gray AM, Abdel-Wahab YH, Flatt PR: The traditional plant treatment, Sambucus nigra (elder), exhibits insulin-like and insulin-releasing actions in vitro. J Nutr 130, 15–20 (2000)

Dhandapani S, Subramanian VR, Rajagopal S, Namasivayam N: Hypolipidemic effect of Cuminum cyminum L. on alloxan-induced diabetic rats. Pharmacol Res 46, 251–255 (2002)

Gokce G, Haznedaroglu MZ: Evaluation of antidiabetic, antioxidant and vasoprotective effects of Posidonia oceanica extract. J Ethnopharmacol 115, 122–130 (2008)

Udayakumar R, Kasthurirengan S, Mariashibu TS, Rajesh M, Anbazhagan VR, Kim SC, Ganapathi A, Choi CW: Hypoglycaemic and hypolipidaemic effects of Withania somnifera root and leaf extracts on alloxan-induced diabetic rats. Int J Mol Sci 10, 2367–2382 (2009)

Bora KS, Sharma A. Phytochemical and pharmacological potential of Medicago sativa: A review. Pharm. Biol. 2011, 49, 211–220.

Rana MG, Katbamna RV, Padhya AA, Dudhrejiya AD, Jivani NP, Sheth NR. In vitro antioxidant and free radical scavenging studies of alcoholic extract of medicago sativa. Rom. J. Biol. – Plant Biol., 2010. Vol 55, No 1, P. 15–22, Bucharest.

Valcheva KS, Kuzmanov K, Mihova V, Krasnaliev I, Borisova P, Belcheva A. Antihyperlipidemic Effect of Aronia Melanocorpa Fruit Juice in Rats Fed a High-Cholesterol Diet. SpringerLink- Journal Article. 2006;30.

Koncazak I, Okuno S, Yoshimoto M,Yamakawa O. Caffeoylquinic Acids Generated In Vitro in High-Anthocyanin-Accumulating Sweet potato Cell Line. Journal of Biomedicine and Biotechnology. 2004;5:287-92

Zern TL, Wood RJ, Greene C, West KL, Liu Y, Aggarwal D, dkk. Grape polyphenol Exert a Cardioprotective Effect in Pre- and postmenopausal Women by Lowering Plasma Lipids and reducing Oxidative Stress. J Nutr. 2005;135:1911

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