Antibacterial Activity Of Δ-Guaiene Patchouli Oil Against Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853

Authors

  • Tania Rizki Fauziah Padjadjaran University

DOI:

https://doi.org/10.21111/agrotech.v6i3.4949

Keywords:

Antibacterial, Δ-guaiene, Escherichia coli, Pseudomonas aeruginosa, Patchouli Oil

Abstract

Patchouli oil known as the main ingredient in fragrances such as perfume, bath soap and hair oil. Not only that, this oil is also known to have antibacterial, antifungal, antiemetic, and trypanocidal activity. One of the highest content of patchouli oil is Δ-guaiene. Δ-guaiene is one of the sesquiterpene groups. The sesquiterpene compounds present in essential oils have microbial and larvicidal activity. The sesquiterpene antibacterials work by affecting the permeability and protein membrane activity of these microorganisms. The purpose of this study was to determine the antibacterial activity of Δ-guaiene of patchouli oil againstEscherichia coli and Pseudomonas aeruginosa. This antibacterial test was carried out by the well diffusion method at various concentrations (20%, 40%, 60%, 80%, and 100% v/v) to determine the diameter of the inhibitory power. The results of this study indicate that the largest zone of inhibition was found at a concentration of 80% for Escherichia coli and 100% for Pseudomonas aeruginosa.

Author Biography

Tania Rizki Fauziah, Padjadjaran University

Tania Rizki Fauziah is currently a student at the Department of Agricultural Engineering in the College of Agricultural Industrial Technology, Padjadjaran University, Sumedang, Indonesia. Her research interests include the postharvest and processing of essential oil such as patchouli oil and antibacterial activity in patchouli oil.

References

Bhaskara, G.Y. 2012.Uji Daya Antifungi Ekstrak Etanol Daun Salam (Syzygium polianthum [Wight] Walp.) Terhadap Candida albicans ATCC 10231 Secara In Vitro. [Skripsi]. Universitas Muhammadiyah Surakarta: Surakarta. CLSI. 2016. Performance Standards forAntimicrobial Susceptibility Testing.26th ed. Wayne: Clinical and Laboratory Standards Institute. 32pp. Elifah, E. 2010. Uji Antibakteri Fraksi Aktif Ekstrak Metanol Daun Senggani (Melastoma candidum, D. Don) Terhadap Escherichia coli dan Bacillus subtilis Serta Profil Kromatografi Lapis Tipisnya. [Skripsi]. FMIPA Universitas Sebelas: Surakarta. Faizah, N., dan Djati, M. S. 2014.Pengaruh Ekstrak Daun Elephantopus scaber.L dan Polyscias obtusa Terhadap Modulasi Sel T CD8+ dan CD8+CD62L+ Mencit Balb/c. Jurnal Biotropika 2 (3): 184-153. Jasmansyah, Fitriyani, P., Sujono, H., dan Aisyah L., S. 2020.Uji Aktivitas Antimikroba Minyak Atsiri Tanaman Pegagan (Centella asiatica (L.) Urb). Jurnal Kartika Kimia 3 (1): 43-47. Mukti, A., Rastina, Harris, A., Ismail, Darniati, dan Masyitha, D. 2017. Resistensi Escherichia coli Terhadap Antibiotik Dari Daging Ayam Broiler Di Pasar Rukoh. Jurnal Ilmiah Mahasiswa Veteriner 1 (3): 492-498. Pelczar, M.J. Dan Chan, E.S.C. 2008.Dasar-dasar mikrobiologi. UI Press: Jakarta. Pratiwi, R., H. 2017. Mekanisme Pertahanan Bakteri Patogen Terhadap Antibiotik. Jurnal Pro-Life 4 (3): 418-429. Rukmono, P dan Zuraida, D. 2013.Uji Kepekaan Antibiotik Terhadap Pseudomonas Aeruginosa Penyebab Sepsis Neonatorum. Sari Pediatri 14 (5): 332-6. Sari, E., M., Maruf, W., F, dan Sumardianto.2014. Kajian Senyawa Bioaktif Ekstrak Teripang Hitam (Holothuria edulis) Basah dan Kering Sebagai Antibakteri Alami. Jurnal Pengolahan dan Bioteknologi Hasil Perikanan 3 (4): 16-24. Swamy, Mallapa K., Mohd, S., and Uma, R. 2016. Antimicrobial Properties of Plant Essential Oil Against Human Pathogens and Their Mode of Action: an updated review. Evidence-Based Complementary and Alternative Medicine 2016. Utami, R., Nurhartadi, E., dan Putra, A., Y., T. 2013.Pengaruh Penambahan Minyak Atsiri Kunyit Putih (Kaempferia rotunda) Pada Edible Film Pati Tapioka Terhadap Aktivitas Antimikroba dan Sensoris. Jurnal Teknologi Hasil Pertanian 2 (2): 51-56. Winarno, S., Maruf, W., F., dan Dewi, E., N. 2012.Uji Bioaktifitas Ekstrak Gelidium sp. Terhadap Bakteri Escherichia coli dan Staphylococcus aureus. Jurnal Perikanan. 1 (2): 1-9.

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Published

2020-11-25

How to Cite

Fauziah, T. R. (2020). Antibacterial Activity Of Δ-Guaiene Patchouli Oil Against Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853. Gontor Agrotech Science Journal, 6(3), 393–402. https://doi.org/10.21111/agrotech.v6i3.4949