Pendugaan Viabilitas benih padi (Oryza sativa L.) dengan Uji Tetrazolium

Viability of Rice Seeds (Oryza sativa L.) Via The Tetrazolium Test

Authors

  • Wati Tri Utami Program Studi Teknologi Benih, Jurusan Pertanian, Politeknik Pembangunan Pertanian Yogyakarta Magelang
  • Asih Farmia Program Studi Teknologi Benih, Jurusan Pertanian, Politeknik Pembangunan Pertanian Yogyakarta Magelang
  • Suharno Suharno Program Studi Teknologi Benih, Jurusan Pertanian, Politeknik Pembangunan Pertanian Yogyak

DOI:

https://doi.org/10.21111/agrotech.v10i1.10830

Keywords:

Uji Tetrazolium, Uji Perkecambahan, Viabilitas Benih

Abstract

Supporting factors for the success of rice planting include seed availability in sufficient quantities, high quality, and availability when needed. Conventional rice seed viability testing generally takes quite a long time, around 14 days. Therefore, it is necessary to develop several methods for quickly testing seed viability. This research aims to determine the coloring pattern of rice seeds, differences in viability tested with 0.25% tetrazolium concentration, and the relationship between the viability values of seeds tested for sprouting and those tested with 0.25% tetrazolium. This research was carried out from March to May 2023 at the Laboratory of the Central Java Seed Supervision and Certification Center. The treatments used were tests using the direct sprout test (rolled paper in plastic test) and the 0.25% concentration tetrazolium test method. The seeds used were seeds with different storage times (0, 1, 2, and 3 months) to obtain variations in seed vigor. The data obtained were analyzed using visual and descriptive analysis, t-test, and correlation analysis. The results showed that the tetrazolium test with a concentration of 0.25% on Inpari 42 rice seeds produced seven coloring patterns grouped into four viable seed and three non-viable seed coloring patterns. The 0.25% concentration tetrazolium test showed that the results had no significant effect and were closely related the results of the direct germination test so the rapid test could be used as a substitute for the (conventional) germination test on a wide scale.

References

AOSA/SCST. (2014). Association of Official Seed Analysts and Society of Commercial Seed Technologists. Rules for Testing Seeds. v.1. Washington DC.

BPS. (2022). Produksi Padi pada 2022 mengalami Kenaikan sebesar 1,25 juta Ton GKG. https://www.bps.go.id/pressrelease/2022/10/17/1910/pada-2022--luas-panen-padi-diperkirakan-sebesar-10-61-juta-hektare-dengan-produksi-sekitar-55-67-juta-ton-gkg.html (diakses pada 20 Februari 2023)

Dwipa, I., Muhsanati, & Wulandari, Y. (2018). Effect of Different Seed Water Content and Storage Duration on Seed Viability of Local Genotype Brown Rice Daro Merah. JERAMI (Indonesian Journal of Crop Science), 9-18.

Eviliani, U. (2016). Uji Tetrazolium untuk Kriteria Vigor Benih Cabai (Capsicum annum). in Institut Pertanian Bogor. Bogor.

Fachruri, M., Muhidong, J., & Sapsal, T., M. (2019). Analisis Pengaruh Suhu dan Kelembaban Ruang terhadap Kadar Air Benih Padi di Gudang Penyimpanan PT. Sang Hyang Seri. Jurnal Teknologi Pertanian. 12(2):136

FranÇa, J. B., dan Krzyzanowski, F. C. (2019). Tetrazolium: an Importante Test for Physiological Seed Quality Evaluation. Journal of Seed Science 41(3): 359-366.

Hasrawati, Mustaril, K., & Dachlan, A. (2015). Pengujian Viabilitas Benih Kacang Tanah (Arachis Hypogeae L.) pada Berbagai Lama Penyimpanan dengan Menggunakan Uji Tetrazolium. Jurnal Agrotan 1(2):94-107

Insan Novita. (2000). Pendugaan Viabilitas Benih Acacia crassicarpa A. Cunn. Ex Benth. Berdasarkan Uji Perkecambahan, Tetrazolium Topografis dan Hidrogen Peroksida in Institut Pertanian Bogor. Bogor.

Kamanga, B.M., E.R. Palupi, E. Widajati, S. Ilyas. (2021). Development of seed vigor testing method using single count of radicle emergence for true seed of shallot (Allium ascalonicum B.) Internat. J. Sci. Food Agric. 5:152-162

Kolo, E., dan Tefa, A. (2016). Pengaruh Kondisi Simpan terhadap Viabilitas dan Vigor Benih Tomat. Savana Cendana 1(3): 112-115.

Martinez, A.C., Pluskota, W.E., Bassel, G.W., Asahina, M., Yamaguchi S, Nonogaki H. (2012). The mechanisms of hormonal regulation of endosperm cap-specific gene expression in tomato seeds. Seed biology in the 21st century: perspectives and new directions. The Plant Journal. 71, 575-586.

Nurzalina. (1995). Penentuan Kriteria Penilaian Viabilitas Benih Swietenia macrophylla King. Berdasarkan Pewarnaan dan Hasil Uji Cepat dengan Tetrazolium. In Institut Pertanian Bogor. Bogor

Paiva, C.T.C; Silva, J.B.; Dapont, E.C.; Alfes, C.Z; Carvalho, M.A.C. (2016). Qualidade Fisiológica e Sanitária de Sementes Comerciais de Alface e Repolho. Revista de Ciências Agroambientais 14(1): 53-59

Rahayu, Siti., Yeyen, Prestyaning., Kobarsih Mahargono. (2011). Penyimpanan Benih Padi Menggunakan Berbagai Jenis Pengemas. Jurnal Agrin. 15(1). 36-42

Salazar, S., Quintero, J. Dan Rojas, J. (2020c). Optimalisasi Uji Tetrazolium pada Tiga Spesies Anggrek Hutan Andes. Australian Journal of Crop Science. 14(5). 822-830

Tola, C. C., Ninacanchi, J. C. H., dan Choque, D.C. (2019). Determinacion de la Concentratracion de Tetrazolio y Tiempo de tincion adecuado para el analisis de Viabilidad en Semillas de cafe. Apthapi.5(3). 171-1682

Zanzibar, M, dan Naning, Y.(2003). Standar prosedur dan kunci interprestasi beberapa benih tanaman hutan berdasarkan uji cepat. Prosiding seminar hasil-hasil penelitian Balai Litbang Teknologi Perbenihan. Badan Penelitian dan Pengembangan Hutan. Departemen Kehutanan. Bogor.

Downloads

Published

2024-05-23

How to Cite

Tri Utami, W., Farmia, A., & Suharno, S. (2024). Pendugaan Viabilitas benih padi (Oryza sativa L.) dengan Uji Tetrazolium: Viability of Rice Seeds (Oryza sativa L.) Via The Tetrazolium Test . Gontor Agrotech Science Journal, 10(1), 1–10. https://doi.org/10.21111/agrotech.v10i1.10830

Issue

Vol. 10 No. 1 (2024): Juni 2024

Section

Articles

License

The author whose published manuscript approved the following provisions:

    1. The right of publication of all material published in the journal / published in the Gontor AGROTECH Science Journal is held by the editorial board with the knowledge of the author (moral rights remain the author of the script).
     2. The formal legal provisions for access to digital articles of this electronic journal are subject to the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (CC BY-NC-SA 4.0), which means that Gontor AGROTECH Science Journal reserves the right to save, transmit media or format, Database), maintain, and publish articles without requesting permission from the Author as long as it keeps the Author's name as the owner of Copyright.
     3. Printed and electronic published manuscripts are open access for educational, research and library purposes. In addition to these objectives, the editorial board shall not be liable for violations of copyright law.