Effect of extraction method on total phenolic content and antioxidant activity of Terminalia catappa (L.) leaves

Authors

  • Tubagus Akmal Akademi Farmasi Bumi Siliwangi, Bandung
  • Andi Ika Julianti Akademi Farmasi Bumi Siliwangi, Bandung
  • Yenni Puspita Tanjung Akademi Farmasi Bumi Siliwangi, Bandung
  • Pipyt Mutiara Akademi Farmasi Bumi Siliwangi, Bandung
  • Sapitri Febriyanti Akademi Farmasi Bumi Siliwangi, Bandung

DOI:

https://doi.org/10.21111/pharmasipha.v8i2.11363

Abstract

The potential for encountering free radicals poses a significant concern. Ketapang (Terminalia catappa L.) is a plant with antioxidant properties due to secondary metabolites, specifically phenolic compounds. The objective of this study was to determine the total phenolic content and antioxidant activity of ketapang leaves through the utilization of several extraction techniques. The extraction of Ketapang leaves is conducted using various methods, namely maceration, stirring-assisted extraction (SAE), ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE) with 1:10 solid-solvent ratio. The total phenolic content of the Ketapang leaf extract was determined using the Folin-Ciocalteu technique and quantified using a UV-Visible spectrophotometer set at a wavelength of 782nm. Meanwhile, antioxidant activity measurements were carried out using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and measured at a wavelength of 516nm. The study investigated the total phenolic content and antioxidant activity of Ketapang leaf extract using the maceration method of stirring-assisted extraction (SAE), ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE), respectively, were 68.495%+2.891, 75.709%+0.106, 84.269%+0.159, and 65.065%+3.965 mgGAE/g and 20.891, 17.569, 15.427, and 21.353 µg/mL. The findings of this study indicate that the ultrasound-assisted extraction technique exhibits the greatest overall phenolic content and antioxidant activity.   Keywords: antioxidant, Terminalia catappa, total phenolic content, ultrasound-assisted extraction

Downloads

Published

2024-08-29

Issue

Vol. 8 No. 2 (2024): September

Section

Articles

License

Copyright (c) 2024 Author

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Authors who publish with this Journal agree to the following terms:

  1. Author retain copyright and grant the journal right of first publication with the work simultaneously licensed under a creative commons attribution license that allow others to share the work within an acknowledgement of the work’s authorship and initial publication of this journal.
  2. Authors are able to enter into separate, additional contractual arrangement for the non-exclusive distribution of the journal’s published version of the work (e.g. acknowledgement of its initial publication in this journal).
  3. Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on their websites) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published works.