Use of Bacillus firmus E65-Talc Based Formulation for The Management of Bacterial Leaf Blight Disease

Authors

  • yadi - suryadi Lab Microbiology ICABIOGRAD
  • Dwi Ningsih susilowati ICABIOGRAD
  • I Made - Samudra ICABIOGRAD

DOI:

https://doi.org/10.21111/agrotech.v7i2.5993

Keywords:

bacterial leaf blight, B. firmus E65, rice, talc-powder formulation

Abstract

It has long been known that bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo), is major bacterial diseases which occured widespread in rice growing countries. This study was performed to determine effect of biological control using endophyte bacteria formulated in talc-based powder against Xoo in rice agroecosystem which grown under organic system of rice intensification (SRI) in Cianjur-West Java. Carriers formulation of talc-based powder was prepared to contain endophytic bacteria of Bacillus firmus (isolate E65) which was previously isolated from the rice field. BLB disease suppression was ranged 2.14% to 97.15%. among cultivars tested, with the average BLB severity among six cultivars was 9.79%. The cv. Inpari 10 (Moderate) showed the lowest disease severity compared with that of resistance cv. Sintanur, Mekongga and Code. This suggests that the biocontrol efficacy of formulation B. firmus E65-talc-based powder was not affected by cultivar susceptibility. The antagonistic bacteria formulation yields up to 9%. The highest yields for each respective treatment treated with talc based-bacterial formulation were obtained using cv. Inpari 10 and Cisantana. The scale-up of biocontrol products that prolongs biocontrol activity may challenged with shelf life of formulation. This study showed advantages of single cultures of bacterial antagonist used in a talc-based formulation is possible to suppress BLB disease and promotes rice yield under SRI field condition.  

Author Biographies

yadi - suryadi, Lab Microbiology ICABIOGRAD

biochem

Dwi Ningsih susilowati, ICABIOGRAD

Lab Microbiology

I Made - Samudra, ICABIOGRAD

Biochem

References

Ardakani, S.S., A. Heydari., N. Khorasani, and R. Arjmandi. 2010. Development of New Bioformulations of Pseudomonas Fluorescens and Evaluation of These Products Against Damping-Off of Cotton Seedlings. Journal of Plant Pathology 92: 83-88. Retrieved from http://www.sipav.org/main/jpp/volumes/0110/011008.pdf Cruz, C.V., J. Bai., I. Ona., H. Leung., R.J. Nelson., T.W. Mew, and J.E. Leach. 2000. Predicting Durability of Disease Resistance Gene Based on an Assessment of The Fitness Loss and Epidemiological Consequences of Avirulence Gene Mutation. PNAS 97(25): 13500-13505 Darmawan, M. 2016. Analysis of Legowo Row Planting System and System of Rice Intensification (SRI) of Paddy Field (Oryza Sativa L.) Toward Growth and Production. Agrotech Journal 1(1) : 14-8. Retrieved from https://doi.org/10.31327/atj.v1i1.202 El-Hassan, S.A. and S.R.Gowen. 2006. Formulation and Delivery of the Bacterial Antagonist Bacillus subtilis for Management of Lentil Vascular Wilt Caused by Fusarium oxysporum f.sp. lentis Journal of Phytopathology 154(3):148–155. DOI: 10.1111/j.1439-0434.2006.01075.x Fravel, D.R. 1988. Role of Antibiosis in the Biocontrol of Plant Disease. Annual Review Phytopathology 26: 75-91. Retrieved from https://www.annualreviews.org/doi/abs/10.1146/annurev.py.26.090188.000451 Furlong, M.J., H.J. Kim., W.S. Pak., K.C. Jo., C.I. Ri., and M.P. Zalucki. 2008. Integration of Endemic Natural Enemies and Bacillus thuringiensis to Manage Insect Pests of Brassica Crops in North Korea. Agriculture Ecology Environment 125:223-238. Hifni, H. and M.K. Kardin. 1998. Grouping of Xanthomonas oryzae pv. oryzae Isolates Using IRRI Near-Isogenic Lines of Rice. Hayati 5(3):66-72. https://repository.ipb.ac.id/bitstream/handle/123456789/29656/Hartini_RH_pengelompokan_isolat1998no3-6672.pdf?sequence=1&isAllowed=y IRRI. 2013. Standard Evaluation System for Rice. IRRI, Manila, the Philippines. Jochum, C.C., L.E. Osborne., and G.Y. Yuen. 2006. Fusarium Head Blight Biological Control With Lysobacter enzymogenes Strain C3. Biological Control 39 (3): 336-344. DOI: 10.1016/j.biocontrol.2006.05.004 Kadir, T.S., Y. Suryadi., A. Guswara., A. Ruskandar, and I. Las. 2006. Influence of Integrated Crop Management Practises on Rice Diseases Occurence. In: Proc. of the Asian Conf. on Emerging Trends in Plant-Microbe Interactions (Gnanamanickam SS, Balasubramanian R and Anand N. Eds.). Center for Advanced Studies in Botany. Univ. of Madras, Chennai, India pp. 128-136. Kadir, T.S., Y. Suryadi, and A.Ruskandar. 2007. Bacterial Blight of Upland Rice. In: Proc. 3rd Asian Conf. Plant Pathol: The Role of Plant Pathology in Rapidly Globalizing Economies of Asia. Fac. of Agric. UGM, Yogyakarta. Kilic. E.O. and G.Y. Yuen. 2004. Comparison of Strains of Lysobacter enzymogenes and PGPR for Induction of Resistance Against Bipolaris sorokiniana in Tall Fescue. Biological Control 30: 446-455. DOI: 10.1016/j.biocontrol.2004.01.014 Kita, N., T.Ohya., H. Oekusa., K.Nomura., M. Manago. and M. Shoda. 2005. Biological Control of Damping-off of Tomato Seedlings and Cucumber Phomopsis root rot by B. subtilis RB 14-C. JARQ 39(2):109-114. Retrieved from https://pdfs.semanticscholar.org/ade8/fc85b1ad14ca143052ca669080f2fba83d40.pdf Mishra, J., and N. Arora. 2016. Bioformulations for Plant Growth Promotion and Combating Phytopathogens: A Sustainable Approach. In: Bioformulations: for Sustainable Agriculture. Arora N.K. et al. (Eds.), Springer India. DOI 10.1007/978-81-322-2779-3_1 Munteanu, A.R. 2014. The Risk Management of Organic Products Along the Value Chain. International Conference on Production Research – Africa, Europe and Middle East 3rd International Conference on Quality and Innovation in Engineering and Management. Nalisha, I., M. Muskhazli. and T. Norfarizan. 2006. Production of bioactive compounds by. B. subtilis against Sclerotium rolfsii. Malaysian Journal of Microbiology 2(2): 19-23. Retrieved from https://mjm.usm.my/uploads/issues/121/research4.pdf Nurhayati, A., W. Lili., T. Herawati., and I. Riyantini. 2015. Derivatif Analysis of Economic and Social Aspect of Added Value Minapadi (Paddy-Fish Integrative Farming) A Case Study in the Village of Sagaracipta Ciparay Sub District, Bandung West Java Province, Indonesia. In: Proceedings of the Aquatic Procedia of 2nd International Symposium on Aquatic Products Processing and Health: 2015 Sep 13-15; Diponegoro University, Semarang: Indonesia; 7:12-18. DOI: 10.1016/j.aqpro.2016.07.002 Oka, I.N. 1997. Integrated Crop Pest Management with Farmer Participation in Indonesia. In: Reasons for Hope: Instructive Experiences in Rural Development, (Anirudh Krishna, Norman Uphoff and Milton Esman, Eds), West Hartford, CT: Kumarian Press. pp. 184-199. Pfiffner, L. and P. Mader. 1997. Effects of Biodynamic, Organic and Conventional Production Systems on Earthworm Populations. Biological Agriculture and Horticulture 15: 2-10 DOI: 10.1080/01448765.1997.9755177 Radulovic, N., P.D. Blagojevic., Z.S .Radic., and N.M. Stojanovic. 2013. Antimicrobial Plant Metabolites: Structural Diversity and Mechanism of Action. Current Medicinal Chemistry 20(7): 932-952 Savary, S., L. Willocquet., F.A.Elazegui., N.P. Castilla. and P.S. Teng. 2000. Rice Pest Constraints in Tropical Asia: Quantification of Yield Losses due to Rice Pests in A Range of Production Situations. Plant Disease. 84:357−369. Retrieved From https://Doi.Org/10.1094/PDIS.2000.84.3.341 Saikia, R., R. Kumar., D.K. Arora., D.K. Gogoi. and P.Azad. 2006. P. aeruginosa Inducing Rice Resistance Against R. solani: Production of Salicylic Acid and Peroxidase. Folia Microbiology. 51(5):375-380. DOI: 10.1007/BF02931579. Schisler, D.A, P. J. Slininger., R. Behle., and M.A.Jackson. 2004. Formulation of Bacillus spp. for Biological Control of Plant Diseases. Phytopathology 94(11):1267-71 DOI: 10.1094/PHYTO.2004.94.11.1267 Stoleru, V.V., N.C. Munteanu., C.M. Stoleru., V. Rotaru., and G. Liliana. 2012. Cultivar Selection and Pest Control Techniques on Organic White Cabbage Yield. Notulae Botanicae Horti Agrobotanici 40(2):190-196. Retrieved from 7993-Manuscript-30150-1-10-20121105.Pdf Suryadi, Y., D.N. Susilowati., A.Akhdiya., T.S. Kadir., and B. Wibowo. 2013a. Efficacy of Consortium Bacteria for Control Rice Diseases Under System of Rice Intensification (SRI) in West Java-Indonesia. Albanian Journal of Agricultural Science 12 (1): 143-147. Retrieved from http://ajas.ubt.edu.al/wp-content/uploads/2018/05/13023yadi.pdf Suryadi, Y., D.N. Susilowati., T.S. Kadir., Z.R. Zaffan., N. Hikmawati. and N.R. Mubarik. 2013b. Bioformulation of Antagonistic Bacterial Consortium for Controlling Blast, Sheath Blight and Bacterial Blight Diseases on Rice. Asian Journal of Plant Pathology 7 (3):92-108, DOI: 10.3923/ajppaj.2013.92.108 Suryadi ,Y., I.M. Samudra., T.P. Priyatno., D.N. Susilowati., P. Lestari., Fatimah, and T.S.Kadir. 2016. Determination of Pathotypes from Indonesian Xanthomonas oryzae pv. oryzae Population Causing Bacterial Leaf Blight and Their Reactions on Differential Rice. Makara Journal of Science 20(3) : 109-118. DOI: 10.7454/mss.v20i3.6241 Uphoff, N. 2003. Higher Yields With Fewer External Inputs? The System of Rice Intensification and Potential Contributions to Agricultural Sustainability. International Journal of Agricultural Sustainability, 1(1):38-50. DOI: 10.3763/ijas.2003.0105 Vasudevan., P.S. Kavitha., P.B. Priyadarisini., L.Babujee., and S.S. Gnanamanickam. 2002. Biological Control of Rice Diseases. In: Biological Control of Crop Diseases. (Gnanamanickam, Ed.). Marcel Decker, New York. pp.11-32. Velusamy. P., J.E. Immanuel., and S.S. Gnanamanickam. 2006. Biological Control of Rice Bacterial Blight by Plant-Associated Bacteria Producing 2,4-Diacetylphloroglucinol. Canadian J.ournal of Microbiology. 52(1):56-64. DOI: 10.1139/w05-106. Vidhyasekaran, P., and M. Muthuamilan. 1995. Development of Formulation of Pseudomonas flourescens for Control of Chickpea Wilt. Plant Disease. 79 (8): 780–782. Yuttitham, M. 2019. Comparison of Carbon Footprint of Organic and Conventional Farming of Chinese Kale. Environment and Natural Resources Journal 17(1): 78-92. Retrieve from.Comparison_of_Carbon_Footprint_of_Organic_and_Conv.pdf Zahri, I., Sabaruddin., M.U. Harun., D.Adriani. and E. Wildayana. 2018. Comparing Rice Farming Appearance of Different Agroecosystem in South Sumatra, Indonesia. Bulgarian Journal of Agricultural Science 24(4):189-198. Retrieved from https://www.agrojournal.org/24/02-03.pdf

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Published

2021-12-28

How to Cite

suryadi, yadi .-., susilowati, D. N., & Samudra, I. M. .-. (2021). Use of Bacillus firmus E65-Talc Based Formulation for The Management of Bacterial Leaf Blight Disease. Gontor Agrotech Science Journal, 7(2), 183–214. https://doi.org/10.21111/agrotech.v7i2.5993

Issue

Vol. 7 No. 2 (2021): December 2021

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