Isolasi Bakteri Simbion Rayap Sebagai Agens Hayati Penyakit Rebah Semai Pada Tanaman Kedelai (Sclerotium Rolfsii) Secara In Vitro

Isolation of Termite Symbion Bacteria as Biological Agents of Seedling Collapse Disease Soybean Plants (Sclerotium rolfsii) in vitro

Authors

  • Vernanda Hani Pradana Sakti Nanda Politeknik Negeri Jember
  • Ardeva Duta Widura Jurusan Produksi Pertanian, Politeknik Negeri Jember
  • Achmad Diva Maulana Jurusan Produksi Pertanian, Politeknik Negeri Jember
  • Zattury Alda Wiya Jurusan Produksi Pertanian, Politeknik Negeri Jember
  • Risma Azizatur Rofiqoh Jurusan Produksi Pertanian, Politeknik Negeri Jember
  • Trisnani Alif Jurusan Produksi Pertanian, Politeknik Negeri Jember
  • Gallyndra Fatkhu Dinata Jurusan Produksi Pertanian, Politeknik Negeri Jember

Keywords:

Agens Hayati, Bakteri Simbion, Kedelai, Rayap, Rebah Semai

Abstract

Termite symbiotic bacteria have potential as a biological agent that has not been extensively researched. This research aims to uncover the potential of termite symbiotic bacteria as a biological agent against the damping off disease (Sclerotium rolfsii) in soybean plants. The research was conducted in the plant protection laboratory, biosciences laboratory, and innovation garden of Jember State Polytechnic over 4 months. The study was designed based on a Completely Randomized Design (CRD) with 6 treatments, each repeated 4 times. The treatments included: P1 pathogenic fungus as control, P2-P4 pathogen with termite symbiotic bacteria with the highest inhibition percentage, and P6 pathogen S. rolfsii. The stages of the research began with the Isolation of Termite Symbiotic Bacteria, Antagonistic Screening Test, Hypersensitivity Test, and In Vitro Antagonistic Test. From the results of testing four isolates, the ability to inhibit the pathogen S. rolfsii was observed. These isolates are IR1A1, IR1A4, IR1A6, and IR4D2. The effectiveness of inhibiting S. rolfsii ranged from 28.97% to 95.79% in vitro. The most effective bacterial isolate in suppressing the pathogen and capable of surpassing fungicide treatment is isolated IR1A6, which has an inhibition rate of up to 95.79%.

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Published

2024-12-25

How to Cite

Nanda, V. H. P. S., Widura, A. D. ., Maulana, A. D., Wiya, Z. A. ., Rofiqoh, R. A. ., Alif, T. ., & Dinata, G. F. . (2024). Isolasi Bakteri Simbion Rayap Sebagai Agens Hayati Penyakit Rebah Semai Pada Tanaman Kedelai (Sclerotium Rolfsii) Secara In Vitro: Isolation of Termite Symbion Bacteria as Biological Agents of Seedling Collapse Disease Soybean Plants (Sclerotium rolfsii) in vitro. Gontor Agrotech Science Journal, 10(2), 157–166. Retrieved from https://ejournal.unida.gontor.ac.id/index.php/agrotech/article/view/13110

Issue

Vol. 10 No. 2 (2024): December 2024 (In Progress)

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