POTENSI DAN KARAKTERISASI ENZIM SELULASE MIKROBA ASAL LIMBAH INDUSTRI MINYAK KAYU PUTIH
DOI:
https://doi.org/10.21111/atj.v3i2.3867Keywords:
limbah minyak kayu putih, biomasa, bioetanolAbstract
Selulase adalah enzim kompleks yang memotong secara bertahap rantai selulosa menjadi glukosa. Enzim selulase banyak dihasilkan dari berbagai jenis mikroba baik bakteri atau kapang indigenous dari biomassa limbah pertanian, salah satunya berasal dari biomassa limbah minyak kayu putih. Salah satu pemanfaatan enzim selulase adalah diguanakan pada proses biokonversi biomassa lignoselulosa pertanian menjadi bioetanol. Penelitian ini Penelitian ini bertujuan untuk mengisolasi mikroba selulolitik dari biomassa limbah industri minyak kayu putih.Hal ini merupakan dasar pada pemanfaatan selulase tersebut dapat dimanfaatkan untuk degradasi biomassa limbah minyak kayu putih menjadi bietanol. Berdasarkan hasil analisa komponen selulosa limbah minyak kayu putih didapatkan kandungan selulosa sebesar 32,37%. Hasil isolasi mikroba asal biomassa minyak kayu putih didapatkan sembilan isolat potenisn selulolitik dengan nilai indek selulolitik terbesar pada isolat C9. Isolat C9 memiliki waktu optimum produksi enzim pada jam ke-16 masa pertumbuhan dari mikroba tersebut. Hasil hidrolisis enzimatik selama satu jam, biomassa minyak kayu putih dengan menggunakan enzim selulase menghasilkan derajat polimerasi sebesar 2,6References
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Noura El-Ahmady El-Naggar, A. A. Sherief, Hamza, S.S. (2011). Bioconversion process of rice straw by thermotolerant cellulolytic Streptomyces viridiochromogenes under solid-state fermentation conditions for bioethanol production. African Journal of Biotechnology 10(56): 11998-12011. Doi 10.5897/AJB11.1256
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Sanz ML, Gibson GR, Rastall RA. (2005). Influence of dossaccaride structure on prebiotic selectivity in vitro. J Agric Frood Chem. 53: 5192-5199. doi:10.1021/jf050276w.
Schwarz. (2001). The cellulosome and cellulose degradation by anaerobic bacteria.Applied Microbiology and Biotechnology, 56(5-6), 634–649
Sukumaran R. K., R. R. Singhania, and A. Pandey, (2005). Microbial cellulasesproduction, applications and challenges,Journal of Scientific and Industrial Research, 64 (11): 832– 844
Wardani1 A, Ahmad S, Hari S. (2015). Keragaman Koloni Bakteri Indigenous Pengolahan Limbah Cair Industri Penyamakan Kulit.J.Biosaintropis. 1(1) : 19-25.
Widyastuti, S.M. (1999). Dekomposisi Limbah Kayu Putuh Menggunakan Trichoderma sp. Terpilih. Buletin Kehutanan 39 : 1-11.
Yan-Ling Liang, Z. Zhang, Min Wu, Yuan Wu, and Jia-Xun Feng. (2014). Isolation, Screening, and Identification of Cellulolytic Bacteria from Natural Reserves in the Subtropical Region of China and Optimization of Cellulase Production by Paenibacillus terrae ME27-1. Biomed Res Int.: 512497. doi: 10.1155/2014/512497
Zahid M, Abbasi M.K, Sohail H dan Nasir R. (2015). Isolation and identification of indigenous plant growth promoting rhizobacteria from Himalayan region of Kashmir and their effect on improving growth and nutrient contents of maize (Zea mays L.).Frontiers in Microbiology. Vol 6 : 1–10
[AOAC].Association of Official Analytical Chemist. (2005). Official Methods of Analysis. The 4th Ed. Gaithersburg, Md: AOAC International
Fitriani, S. Bahri dan Nurhaeni. (2013). Produksi Bioetanol Tongkol Jagung (Zea Mays) dari Hasil Proses Delignifikasi.Online Jurnal of Natural Science, Vol 2 (3) : 66-74
Harshvardhan K, Mishra A, Jha B. (2013). Purification and characterization of cellulase from a marine Bacillus sp. H1666: a potential agent for single step saccharification of seaweed biomass. Mol Catal Enz. 93: 51-56. doi: 10.1016/j.molcatb.2013.04.009
Ire, F.S., Ezebuiro, V.Ogugbue, C.J. Bioresour. (2016). Production of bioethanol by bacterial co-culture from agro-waste-impacted soil through simultaneous saccharification and co-fermentation ofsteam-exploded bagasse.Bioprocess. 3: 26. doi:10.1186/s40643-016-0104-x
Jeffrey, L.S.H., M.R. Azrizal. (2017). Screening for cellulase activities in actinomycetes isolated from different locations of Peninsular Malaysia. Journal of Tropical Agriculture and Food Science. 35(1): 153–157.
Johnsen, H.R. and Krause, K. (2014). Cellulase Activity Screening Using Pure Carboxymethylcellulose: Application to Soluble Cellulolytic Samples and to Plant Tissue Prints. Int J Mol Sci. 15(1): 830–838.doi: [10.3390/ijms15010830].
Juturu V, Jin Chuan Wu. (2014). Microbial cellulases: Engineering, production and applications. Renewable and Sustainable Energy Reviews 33 : 188–203
Kholis M., N. & Sari, M. (2018). Potensi Biomassa Limbah Pertanian Dalam Produksi Bioetanol. Conference on Innovation and Application of Science and Technology. Universitas Widyagama Malang. 12 September 2018.
Kuhad R.C., R. Gupta, & A.Singh. (2011). Microbial Cellulases and Their Industrial Applications .Enzyme Research, vol. 2011, Article ID 280696, 10 pages,doi:10.4061/2011/280696
Milala, M. A., A. Shugaba, A. Gidado, A. C. Ene, J. A. Wafar. (2005). Studies on the use of agricultural wastes for cellulose enzyme production by A. niger. Journal of Agriculture and Biological Science, 1:325–328.
Novia, I. Utami, L. Windiyati. (2014). Pembuatan Bioetanol Dari Sekam Padi Menggunakan Kombinasi Soaking In Aqueous Ammonia (Saa) Pretreatment – Acid Pretreatment – Hidrolisis – Fermentasi. Teknik Kimia No. 1, Vol. 20.
Noura El-Ahmady El-Naggar, A. A. Sherief, Hamza, S.S. (2011). Bioconversion process of rice straw by thermotolerant cellulolytic Streptomyces viridiochromogenes under solid-state fermentation conditions for bioethanol production. African Journal of Biotechnology 10(56): 11998-12011. Doi 10.5897/AJB11.1256
Pambudiono A, Endang S, Mohamad A. (2018). The Potential of Indigenous Bacteria for Removing Cadmium from Industrial Wastewater in Lawang, East Java.J. Tropical Life Ccience. 8(1) : 62 – 67.
Rachmaniah O, Lisa F.S, Lazuardi K. (2009). Pengaruh liquid hot water terhadapperubahan struktur sel bagas.Makalah pada Seminar Nasional XIV, 22-23 Juli 2009. Surabaya.
Rasul F, A. Afroz, U. Rashid, S. Mehmood, K. Sughra, N. Zeeshan. (2015).Screening and characterization of cellulase producing bacteria from soil and waste (molasses) of sugar industry. International Journal of Biosciences. 6 (3) :230-238
Reddy, K.V. T. Vijayalashmi, P. Ranjit, M.N. Raju. (2017). Characterization of Some Efficient Cellulase Producing Bacteria Isolated from Pulp and Paper Mill Effluent Contaminated Soil. Braz. arch. biol. technol. 60 http://dx.doi.org/10.1590/1678-4324-201716022
Saini, A, N. K Aggarwal, A.Yadav. (2017). Isolation and Screening of Cellulose Hydrolyzing Bacteria from Different Ecological Niches. Bioengineering and Bioscience 5(1): 7-13, 2017 DOI: 10.13189/bb.2017.050102
Sanz ML, Gibson GR, Rastall RA. (2005). Influence of dossaccaride structure on prebiotic selectivity in vitro. J Agric Frood Chem. 53: 5192-5199. doi:10.1021/jf050276w.
Schwarz. (2001). The cellulosome and cellulose degradation by anaerobic bacteria.Applied Microbiology and Biotechnology, 56(5-6), 634–649
Sukumaran R. K., R. R. Singhania, and A. Pandey, (2005). Microbial cellulasesproduction, applications and challenges,Journal of Scientific and Industrial Research, 64 (11): 832– 844
Wardani1 A, Ahmad S, Hari S. (2015). Keragaman Koloni Bakteri Indigenous Pengolahan Limbah Cair Industri Penyamakan Kulit.J.Biosaintropis. 1(1) : 19-25.
Widyastuti, S.M. (1999). Dekomposisi Limbah Kayu Putuh Menggunakan Trichoderma sp. Terpilih. Buletin Kehutanan 39 : 1-11.
Yan-Ling Liang, Z. Zhang, Min Wu, Yuan Wu, and Jia-Xun Feng. (2014). Isolation, Screening, and Identification of Cellulolytic Bacteria from Natural Reserves in the Subtropical Region of China and Optimization of Cellulase Production by Paenibacillus terrae ME27-1. Biomed Res Int.: 512497. doi: 10.1155/2014/512497
Zahid M, Abbasi M.K, Sohail H dan Nasir R. (2015). Isolation and identification of indigenous plant growth promoting rhizobacteria from Himalayan region of Kashmir and their effect on improving growth and nutrient contents of maize (Zea mays L.).Frontiers in Microbiology. Vol 6 : 1–10
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2020-01-19
Published
2019-11-28
How to Cite
Kholis, M. N., Asmediana, A., & Sari, M. (2019). POTENSI DAN KARAKTERISASI ENZIM SELULASE MIKROBA ASAL LIMBAH INDUSTRI MINYAK KAYU PUTIH. Agroindustrial Technology Journal, 3(2), 110–118. https://doi.org/10.21111/atj.v3i2.3867
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