ISOLASI DAN KARAKTERISASI BAKTERI ENDOFIT TANAMAN TOMAT CHERRY (Solanum lycopersicum var. cerasiforme) DALAM KEMAMPUANNYA MENGHASILKAN HORMON ASAM INDOL ASETAT (AIA)

Authors

  • Oktira Roka Aji Program Studi Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Ahmad Dahlan

DOI:

https://doi.org/10.21111/agrotech.v3i1.909

Keywords:

bakteri endofit, tomat cherry, hormon AIA

Abstract

Bakteri endofit adalah bakteri yang hidup didalam tanaman sehat tanpa menyebabkan perubahan morfologi ataupun menimbulkan penyakit. Bakteri endofit membantu meningkatkan kesehatan dan produktivitas tanaman dengan cara menekan bakteri patogen dan meningkatkan pertumbuhan tanaman. Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi kemampuan bakteri endofit yang terdapat pada buah dan batang tanaman tomat cherry (Solanum lycopersicum var. cerasiforme) dalam menghasilkan hormon asam indol asetat (AIA). Hasil proses isolasi bakteri diperoleh 8 isolat bakteri endofit, yaitu 3 isolat dari buah tomat matang (isolat M1, M2, M3), 3 isolat dari buah tomat mentah (isolat Mt1, Mt2 dan Mt3) dan 2 dari batang tanaman tomat cherry (isolat Bt1 dan Bt2). Beberapa bakteri endofit yang berhasil diisolasi memiliki bentuk coccus, bentuk (form) koloni sirkuler, permukaan (elevation) koloni convex, tepi (margin) koloni entire dan berwarna putih. Semua isolat mampu memproduksi hormon AIA kecuali isolat M1 dan Mt3. Bakteri ini berpotensi untuk meningkatkan produktivitas tanaman tomat cherry melalui hormon AIA yang dihasilkan.

References

Bacon, C.W. & Hinton, D.M. 2006. Bacterial endophytes: The endophytic niche, its occupants, and its utility. In Samuel S. Gnanamanickam (Eds), Plant-Associated Bacteria (pp. 155–194). Netherlands : Springer.

Boches, P., Peterschmidt, B., & Myers, J. R. 2011. Evaluation of a Subset of the Solanum lycopersicum var. cerasiforme Core Collection for Horticultural Quality and Fruit Phenolic Content. Hortscience, 46(11), 1450–1455.

Campbell, N.A. & Reece, J.B. 2003. Biology Edisi Kelima. Jakarta: Erlangga

Cohen, J. D. (1996). In Vitro Tomato Fruit Cultures Demonstrate a Role for Indole-3-Acetic Acid in Regulating Fruit Ripening. Journal American Society Horticulture Science, 121(3), 520–524.

Devoghalaere, F., Doucen, T., Guitton, B., Keeling, J., Payne, W., Ling, T.J., Ross, J.J. 2012. A Genomics Approach to Understanding the Role of Auxin in Apple ( Malus X Domestica ) Fruit Size Control. BMC Plant Biology, 12, 7.

Faria, D.C. Dias, A.C.F., Melo, I.S., & Costa, F.E.C. 2013. Endophytic bacteria isolated from orchid and their potential to promote plant growth. World Journal of Microbiology and Biotechnology, 29(2), 217–221. doi:10.1007/s11274-012-1173-4

Gordon, S. A & Weber, R. P. 1951. Colorimetric estimation of asam indol asetat. Plant Physiology, 26, 192-19.

Janda, J Michael, and Sharon L Abbott. 2007. 16S rRNA Gene Sequencing for Bacterial Identification in the Diagnostic Laboratory : Pluses , Perils , and Pitfalls. Journal Of Clinical Microbiology, 45(9), 2761–2764. doi:10.1128/JCM.01228-07.

Ji, S.H., Gururani, M.A. & Chun, S.C. 2014. Isolation and characterization of plant growth promoting endophytic diazotrophic bacteria from Korean rice cultivars. Microbiological Research, 169(1), 83–98. doi: http://dx.doi.org/10.1016/j.micres.2013.06.003

Khan, Z. & Doty, L.S. 2009. Characterization of Bacterial Endophytes of Sweet Potato Plants. Journal Plant Soil, 10, 1-10. doi: 10.1007/s11104-009-9908-1

Latche, A. 2005. The Tomato Aux / AIA Transcription Factor AIA9 Is Involved in Fruit Development and Leaf Morphogenesis. The Plant Cell, 17, 2676–92. doi:10.1105/tpc.105.033415.1.

Malfanova, N. V. 2013. Endophytic bacteria with plant growth promoting and biocontrol abilities. (Dissertation). Leiden University, Netherlands.

Mano, Y. & Nemoto, K. 2012. The pathway of auxin biosynthesis in plants. Journal of Experimental Botany, 63(8), 2853–2872. doi:https://doi.org/10.1093/jxb/ers091

Miliute, I., Buzaite, O., Baniulis, D., Stanys, V. 2015. Bacterial endophytes in agricultural crops and their role in stress tolerance : a review. Zemdirbyste-Agriculture, 102(4), 465–478. doi:10.13080/z-a.2015.102.060

Pavlo, A, Leonid, O., Iryna, Z., Natalia, K., Maria, P.A. 2011. Endophytic bacteria enhancing growth and disease resistance of potato (Solanum tuberosum L.). Biological Control, 56(1), 43–49. doi:http://dx.doi.org/10.1016/j.biocontrol.2010.09.014

Purwanto, U.M.S., Fachriyan H.P., Maria B. 2014. Isolasi Bakteri Endofit dari Tanaman Sirih Hijau ( Piper betle L .) dan Potensinya sebagai Penghasil Senyawa Antibakteri. Current Biochemistry, 1(1), 51–57.

Spaepen, S. & Vanderleyden, J. 2011. Auxin and plant-microbe interactions. Cold Spring Harbor Perspectives in Biology, 3(4), 1–13. doi: 10.1101/cshperspect.a001438

Suhandono, S., & Utari, I. B. 2014. Isolation and Molecular Identification of Endophytic Bacteria from the Arils of Durian (Durio zibethinus Murr) var . Matahari, Microbiology Indonesia, 8(4), 161–169. doi:10.5454/mi.8.4.3

Suhandono, S., Kusumawardhani, M.K. & Aditiawati, P. 2016. Isolation and Molecular Identification of Endophytic Bacteria From Rambutan Fruits (Nephelium Lappaceum L.) Cultivar Binjai. HAYATI Journal of Biosciences, 1–6. doi:10.1016/j.hjb.2016.01.005.

Susilowati DN, Saraswati R, Elsanti & Yuniarti E. 2003. Isolasi dan Seleksi Mikroba Diazotrof Endofitik dan Penghasil Zat Pemacu Tumbuh pada Tanaman Padi dan Jagung. Balai penelitian Bioteknologi dan Sumberdaya Genetik Pertanian, 128- 143.

Tian, B., Cao, Y. & Zhang, K. 2015. Metagenomic insights into communities, functions of endophytes, and their associates with infection by root-knot nematode Meloidogyne incognita. Nature Publishing Group, 1–15. doi: http://dx.doi.org/10.1038/srep17087

Zakiyah, A., Radiastuti, N. & Sumarlin, L.O. 2015. Aktivitas Antibakteri Kapang Endofit dari Tanaman Kina (Cinchona calisaya Wedd). Al-Kauniyah, 8(2), 51–58.

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Published

2017-06-30

How to Cite

Aji, O. R. (2017). ISOLASI DAN KARAKTERISASI BAKTERI ENDOFIT TANAMAN TOMAT CHERRY (Solanum lycopersicum var. cerasiforme) DALAM KEMAMPUANNYA MENGHASILKAN HORMON ASAM INDOL ASETAT (AIA). Gontor Agrotech Science Journal, 3(1), 55–69. https://doi.org/10.21111/agrotech.v3i1.909

Issue

Vol. 3 No. 1 (2017): June 2017

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